A transparent look at purity, production, and Health Canada standards
NMN is one of the most talked-about longevity supplements in Canada because of its role in supporting NAD+ levels. NAD+ fuels cellular energy, metabolism, and healthy aging. But as demand has grown, so has confusion about quality. The truth is simple: not all NMN is equal. The way it is made determines its purity, stability, and effectiveness.
At Zeroo Health, we focus on transparency and evidence-based manufacturing. This guide walks you through exactly how our 500mg NMN is sourced, produced, purified, and tested to meet strict Canadian standards.
1. Sourcing Premium NMN: Why Enzymatic Synthesis Matters
NMN cannot be extracted from food in meaningful quantities, so it must be created in a controlled scientific process. There are three approaches:
Chemical synthesis:
Low quality, low purity, and often contaminated with unwanted by-products.
Fermentation:
More natural but extremely inefficient and expensive.
Enzymatic synthesis (our method):
Clean, precise, and highly pure. This method uses natural enzymes to build NMN the same way the human body does. It produces pharmaceutical-grade, bioidentical NMN without toxic solvents.
Why we use enzymatic synthesis
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Produces >99 percent pure NMN
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Avoids heavy chemicals and harsh solvents
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Delivers a clean, stable, bioidentical form
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Environmentally friendly manufacturing
This method creates NMN molecules with extremely high accuracy, ensuring a clean raw powder even before purification.
2. Manufacturing in Canada: From Raw NMN to 500mg Capsules
Once the NMN is produced, it moves through a carefully controlled, Canadian-regulated manufacturing process.

Step 1: Purification
Chromatography is used to remove water, enzymes, and unreacted ingredients. This creates a stable NMN crystal that resists heat and moisture.
Step 2: Drying and milling
Low-temperature drying protects the molecule from degradation. The powder is then milled into a fine, consistent texture for quick absorption.
Step 3: Encapsulation
Each capsule is filled with exactly 500mg of NMN using calibrated precision equipment. We choose plant-based cellulose capsules for clean labeling and broad dietary compatibility.
3. Health Canada Standards: What Makes Canadian NMN Safer
Many NMN products sold online are unregulated and may contain fillers or mislabeled ingredients. Zeroo Health follows strict Health Canada rules for Natural Health Products.
Natural Product Number (NPN)
Every legitimate supplement in Canada must have an NPN. This means Health Canada has reviewed and approved the product for:
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Safety
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Quality
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Verified ingredients
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Manufacturing standards
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Proper dosage
GMP-certified manufacturing
Our facility operates under federal Good Manufacturing Practices, which control:
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Air filtration and cleanliness
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Staff training
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Batch traceability
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Sanitation protocols
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Ingredient verification
This ensures every bottle meets consistent, pharmaceutical-grade standards.
4. Triple-Layer Testing: Purity and Safety You Can Verify
Zeroo Health uses three independent testing stages to guarantee potency and safety.
1. HPLC purity testing
High-Performance Liquid Chromatography detects any impurities. Zeroo Health NMN consistently measures above 99 percent purity.
2. Heavy metals screening
Every batch is tested for lead, mercury, arsenic, and cadmium at parts-per-billion sensitivity.
3. Microbial analysis
We screen for bacteria, yeast, and mold to ensure the finished product is clean and safe.
Third-party validation
An accredited independent laboratory confirms all results for unbiased verification.
5. Why 500mg NMN Is the Ideal Daily Dose for Canadians
Human clinical trials show that 250 to 1000mg of NMN per day significantly increases NAD+ levels and supports:
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Energy production
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Metabolic function
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Recovery and physical performance
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Healthy aging
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Cellular repair
Lower doses may not produce meaningful results for everyone, while much higher doses often show diminishing returns. This is why we standardized on a single, scientifically supported 500mg dose.
6. Stability and Freshness: Keeping NMN Potent
NMN is sensitive to heat, light, and humidity. To protect it, we use:
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Stabilized NMN crystal form
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Opaque UV-blocking bottles
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Freshness-preserving desiccant packs
These layers keep the product stable at room temperature and prevent breakdown into nicotinamide.
Storage tip
For long-term storage, refrigeration helps maintain maximum potency.
Why Canadians Trust Zeroo Health NMN
Zeroo Health 500mg NMN is designed for purity, safety, and real results backed by strict Canadian oversight. It is:
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NPN-licensed in Canada
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Produced using enzymatic synthesis
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Tested for >99 percent purity
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Verified by third-party labs
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Encapsulated in GMP-certified facilities
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Free from fillers, additives, and solvents
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Vegan, non-GMO, and clean-label
When it comes to longevity supplements, quality is everything. With Zeroo Health, you know exactly what you’re putting into your body and why it works.
Sources
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NMN Manufacturing Methods (Enzymatic vs. Chemical): https://www.nmn.com/news/how-are-nmn-supplements-made
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Scientific Review on NMN Biosynthesis: https://pubs.acs.org/doi/abs/10.1021/acs.jafc.3c09217
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Biosynthesis and Production Review: https://pubmed.ncbi.nlm.nih.gov/38330904/
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Health Canada Licensed Natural Health Products Database (LNHPD): https://www.canada.ca/en/health-canada/services/drugs-health-products/natural-non-prescription/applications-submissions/product-licensing/licensed-natural-health-products-database.html
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Health Canada Good Manufacturing Practices (GMP) Guide: https://www.canada.ca/en/health-canada/services/drugs-health-products/compliance-enforcement/good-manufacturing-practices/guidance-documents/guide-natural-health-products-0158.html
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Long-term NMN Administration Study: https://pmc.ncbi.nlm.nih.gov/articles/PMC5668137/
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Harvard Study on NMN Benefits: https://www.nad.com/news/harvard-study-nmn-cuts-weight-cholesterol-and-blood-pressure-in-overweight-adults
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Dosage and Usage Guide: https://www.nmn.com/taking-nmn